RESUMO
OBJECTIVE: To detect serum hepcidin and erythroferrone levels in child-bearing women with iron deficiency anemia (IDA), and to investigate the association between them and iron status parameters. METHODS: The study consisted of 65 child-bearing women (35 with iron deficiency anemia and 30 age-matched healthy women). The levels of serum iron were detected by using automated chemistry analyzer, the contents of serum ferritin were detected by electrochemiluminescence immunoassay, and the levels of serum erythroferrone and hepcidin were detected by specific enzyme-linked immunosorbent assay (ELISA) kit. The quantitative variables between two groups were compared and analyzed by SPSS22.0 software. Spearman correlation was used to detect correlation between the parameters. RESULTS: The levels of Hb, serum iron, ferritin and transferrin saturation were significantly decreased in IDA patients as compared with in control group (P<0.001). Serum hepcidin levels in IDA patients were significant lower than those in control group (P<0.001). Serum erythroferrone levels slightly increased in IDA group (P>0.05). In IDA patients, serum hepcidin concentrations were positively correlated with hemoglobin concentration, serum iron, serum ferritin and transferrin saturation (r=0.448, r=0.496, r=0.754, r=0.491). But, serum erythroferrone concentrations showed no correlation with hemoglobin concentration, serum iron, serum ferritin, transferrin saturation and hepcidin (P>0.05). CONCLUSION: Serum hepcidin levels were significantly decreased in child-bearing women with IDA, but the serum erythroferrone levels were not obviously different between two groups, suggesting that serum erythroferrone may be not involved in the regulation of iron metabolism in child-bearing women with mild and moderate IDA.
Assuntos
Anemia Ferropriva , Hepcidinas , Criança , Ensaio de Imunoadsorção Enzimática , Feminino , Ferritinas , Humanos , Ferro/metabolismoRESUMO
OBJECTIVE: To detect the expression level of cyclooxygenase-1ï¼COX-1ï¼ and cyclooxygenase-2ï¼COX-2ï¼ in the platelet of iron deficiency anemiaï¼IDAï¼women at childbearing age and to explore its correlation with the different indexes of anemia and platelets. METHODS: Forty female IDA patients at childbearing age and 35 healthy controls were enrolled in this study. The Flow cytometry was used to detect the expression of platelet COX-1 and COX-2ï¼the platelet aggregation function as examined by turbidimetric methodï¼and the levels of serum ferritin were analyzed by electrochemical luminescence methodï¼the leval of serum iron was determined by ELISAï¼and the correlation of different indexes was analyzed. RESULTS: Compared with healthy controlsï¼the levels of platelet COX-1 and COX-2 were significantly lower in female IDA patients at Childbearing ageï¼P<0.05ï¼ï¼but platelet countï¼Pltï¼ï¼mean platelet volumeï¼MPVï¼ and platelet aggregation rateï¼PAgTï¼were not statistically different between the 2 groupsï¼P > 0.05ï¼. The expression level of platelet COX-1 positively correlated with those of Hbï¼r =0.623ï¼P<0.01ï¼ï¼serum ironï¼r =0.321ï¼P<0.05ï¼ and HCTï¼r=0.305ï¼P<0.05ï¼. but the platelet COX-2 expression did not corelate with these indexs. CONCLUSION: The expression of platelet COX-1 and COX-2 in female IDA patients at Childbearing age markedly decreaseï¼and the expression level of platelet COX-1 closely relates with the severity of anemiaï¼that possesses reference value for clinical diagnosis of female IDA patients at Childbearing age..
Assuntos
Anemia Ferropriva , Plaquetas , Ciclo-Oxigenase 1 , Ciclo-Oxigenase 2 , Feminino , Ferritinas , Humanos , Agregação Plaquetária , Contagem de PlaquetasRESUMO
AIM: To investigate the distribution of killer cell immunoglobulin-like receptor (KIR) and its specific ligand human leukocyte antigen (HLA) in Jiangsu Han population. METHODS: 173 samples from unrelated healthy individuals of Jiangsu Han population were genotyped and observed for KIR, HLA-Cw and HLA-Bw4 using a SYBR Green I real-time PCR and PCR-SSP method, respectively. The number and type of KIR/HLA pairs inherited in each individual were analyzed. RESULTS: In Jiangsu Han population, all four inhibitory KIR (2DL1, 2DL2/3, 3DL1 and 3DL2) that recognize the classical HLA class I molecules HLA-A, -B and -C were present in >92% of the study group. Frequencies of 2DL2/HLA-C1, 2DL3/HLA-C1, 2DL1/HLA-C2 and 3DL1/Bw4 were 0.243, 0.971, 0.457 and 0.590, respectively; frequencies of 2DS1/HLA-C2 and 2DS2/HLA-C1 were 0.162 and 0.231, respectively. 54.3% of the cases expressed KIR2DL1 without HLA-C1, 32.9% inherited 3DL1 without HLA-Bw4 and 5.8% expressed HLA-Bw4 without 3DL1. 27.7% of the individuals had three iKIR/HLA pairs, 26% carried two iKIR/HLA pairs, and 25.4% inherited a single iKIR/HLA pair and no one was deficient in all three iKIR/HLA pairs. CONCLUSION: There was disparity between KIR receptor and HLA ligand in Jiangsu Han population. Inhibitory KIR/HLA pair frequency was higher than stimulatory one. About 1/4 of the study group expressed a single iKIR/HLA pair alone.
Assuntos
Receptores KIR/genética , China/etnologia , Frequência do Gene , Técnicas de Genotipagem , Antígenos de Histocompatibilidade Classe I/genética , Antígenos de Histocompatibilidade Classe I/imunologia , Teste de Histocompatibilidade , Humanos , Células Matadoras Naturais/imunologia , Células Matadoras Naturais/metabolismo , Ligantes , Receptores KIR/imunologiaRESUMO
AIM: To develope a novel real-time PCR method for KIR genotyping. METHODS: KIR genotyping is performed using 16 real-time PCR reactions, each containing two-three KIR-specific primers, a pair of internal positive control primers and a fluorescence dye, SYBR Green I. By the analysis of the Tm and the characteristic of the melting curve of the amplified products, we identified the presence or absence of 16 KIR genes. A variety of dilution folds were made to detect the sensitivity of this method. RESULTS: KIR genes were effectively genotyped by the analysis of the melting curve. This method can be used to detect KIR genes even from 0.1 ng of DNA. The feasibility of this method was tested by genotyping 10 DNA samples from Peripheral blood and 10 DNA samples from cervical cell. CONCLUSION: We developed a novel real-time PCR assay with SYBR Green I, which is a simple, rapid, sensitive, real-time and environmental method. It provides the possibility of the automated KIR genotyping.